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The protective effect of nigeglanine on dextran sulfate sodium‐induced experimental colitis in mice and Caco‐2 cells
Author(s) -
Gao Xuejiao,
Tang Bin,
Liang Huihuang,
Yi Li,
Wei Zigong
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28909
Subject(s) - colitis , dextran , caco 2 , chemistry , sulfate , sodium , biophysics , biochemistry , medicine , cell , biology , organic chemistry
Ulcerative colitis (UC) was a nonspecific inflammatory disease. The treatment of UC is imperative. The present study aimed to investigate the effect of nigeglanine on dextran sulfate sodium‐induced UC in experimental mice and Caco‐2 cells and define the underlying mechanism. The nigeglanine was shown a significant protective effect on the colon, significantly reduced the weight and colon length loss and inhibited intestinal epithelial cell damage. Nigeglanine also reduced proinflammatory factors and increased anti‐inflammatory factor production. The results indicate that nigeglanine suppresses the nuclear factor kappa B and mitogen‐activated protein kinases pathways in addition to NLRP3 inflammasome action, inhibiting colon epithelial cell pyroptosis. Surprisingly, ZO‐1 and occludin protein levels increased with nigeglanine treatment, suggesting that nigeglanine plays a protective role in barrier integrity, reducing colitis progression. The present study suggests that dietary therapy with nigeglanine may be a useful treatment for prophylaxis and palliative UC.