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Transcriptome analysis reveals lncRNA‐mediated complex regulatory network response to DNA damage in the liver tissue of Rattus norvegicus
Author(s) -
Huang Chen,
Leng Dongliang,
Lei Kuan Cheok,
Sun Shixue,
Zhang Xiaohua Douglas
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28889
Subject(s) - transcriptome , biology , dna damage , dna , computational biology , liver tissue , microbiology and biotechnology , genetics , gene , gene expression , endocrinology
DNA is prone to damages, which would result in genetic disorders and enhance risk of tumorigenesis. Hence, understanding the molecular mechanisms of DNA damage and repair will provide deep insights into tumorigenesis, carcinogenesis as well as the corresponding treatments. Aiming at investigating potential long noncoding RNAs (lncRNAs) response against DNA damage, we performed a comprehensive transcriptomic analysis based on RNA sequencing data of the liver tissue from Rattus norvegicus , in which DNA damage was induced using aflatoxin B1, ifosfamide and N ‐nitrosodimethylamine. Through our analyses, numerous novel lncRNAs are identified for the first time, and differential network analysis discloses lncRNA‐mediated regulatory networks related to DNA‐damage response. The result shows that these DNA‐damage‐inducing chemicals might disrupt many lncRNA‐mediated interactions involved in diverse biological processes and pathways, for example, immune function and cell adhesion. In contrast, the host might also activate a few RNA interactions in response to DNA damage, involving response to drug and regulation of cell cycle.