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p53/miR‐30a‐5p/ SOX4 feedback loop mediates cellular proliferation, apoptosis, and migration of non‐small‐cell lung cancer
Author(s) -
Quan Xiaowei,
Li Xuelian,
Yin Zhihua,
Ren Yangwu,
Zhou Baosen
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28851
Subject(s) - microrna , sox4 , biology , cell growth , carcinogenesis , chromatin immunoprecipitation , cancer research , apoptosis , western blot , mcl1 , lung cancer , microbiology and biotechnology , cancer , downregulation and upregulation , gene expression , gene , promoter , oncology , genetics , medicine
Many microRNAs (miRNAs) play vital roles in the tumorigenesis and development of cancers. In this study, we aimed to identify the differentially expressed miRNAs and their specific mechanisms in non‐small‐cell lung cancer (NSCLC). Based on data from the GSE56036 database, miR‐30a‐5p expression was identified to be downregulated in NSCLC. Further investigations showed that overexpression of miR‐30a‐5p inhibited cell proliferation, migration, and promoted apoptosis in NSCLC. Increase of miR‐30a‐5p level could induce the increase of Bax protein level and decrease of Bcl‐2 protein level. In addition, chromatin immunoprecipitation assays showed that miR‐30a‐5p expression was induced by binding of p53 to the promoter of MIR30A. Bioinformatics prediction indicated that miR‐30a‐5p targets SOX4, and western blot analysis indicated that overexpression of the miRNA decreases the SOX4 protein expression level, which in turn regulated the level of p53. Thus, this study provides evidence for the existence of a p53/miR‐30a‐5p/SOX4 feedback loop, which likely plays a key role in the regulation of proliferation, apoptosis, and migration in NSCLC, highlighting a new therapeutic target.