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MicroRNA‐181b‐5p modulates tumor necrosis factor‐α‐induced inflammatory responses by targeting interleukin‐6 in cementoblasts
Author(s) -
Wang Xiaoxuan,
Sun Hualing,
Liu Huan,
Ma Li,
Jiang Chenxi,
Liao Haiqing,
Xu Shihan,
Xiang Junbo,
Cao Zhengguo
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28837
Subject(s) - cementoblast , proinflammatory cytokine , cementum , tumor necrosis factor alpha , microbiology and biotechnology , inflammation , chemokine , medicine , immunology , biology , pathology , dentin
Tooth cementum is a bone‐like mineralized tissue and serves as a microbial barrier against invasion and destruction. Cementum is also responsible for tooth stability and defending pulp from outside stimuli, which is formed by cementoblasts. Although it is crucial for periodontal and periapical diseases, the mechanisms underlying the pathophysiological changes of cementoblasts and their inflammatory responses remain unclear. MiR‐181b is found to modulate vascular inflammation and endotoxin tolerance. In this study, miR‐181b‐5p was downregulated in tumor necrosis factor‐α (TNF‐α)‐stimulated cementoblasts, whereas proinflammatory molecules increased. The mouse periapical lesions have similar results, which imitate an inflammatory environment for cementoblasts in vivo. The bioinformatics analysis and dual luciferase reporter assay suggested that miR‐181b‐5p targeted interleukin‐6 ( IL‐6 ). Overexpressing miR‐181b‐5p negatively regulated IL‐6 and proinflammatory chemokine. Western blot analysis and luciferase activity reporter assay verified that miR‐181b‐5p weakened the NF‐κB activity. Hence, miR‐181b‐5p moderated proinflammatory chemokine production by targeting IL‐6 in cementoblasts and NF‐κB signaling pathway was involved. Furthermore, miR‐181b‐5p promoted cementoblast apoptosis, which may enhance the resolution of inflammation. Overall, our data revealed that miR‐181b‐5p was a negative regulator of TNF‐α‐induced inflammatory responses in cementoblasts.