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Spatial control of oxygen delivery to three‐dimensional cultures alters cancer cell growth and gene expression
Author(s) -
Wulftange William J.,
Rose Michelle A.,
GarmendiaCedillos Marcial,
da Silva Davi,
Poprawski Joanna E.,
Srinivasachar Dhruv,
Sullivan Taylor,
Lim Langston,
Bliskovsky Valery V.,
Hall Matthew D.,
Pohida Thomas J.,
Robey Robert W.,
Morgan Nicole Y.,
Gottesman Michael M.
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28665
Subject(s) - matrigel , oxygen , bioreactor , polydimethylsiloxane , cancer cell , cell culture , limiting oxygen concentration , microbiology and biotechnology , cell , biophysics , 3d cell culture , chemistry , biology , nanotechnology , materials science , biochemistry , cancer , botany , genetics , organic chemistry
Commonly used monolayer cancer cell cultures fail to provide a physiologically relevant environment in terms of oxygen delivery. Here, we describe a three‐dimensional (3D) bioreactor system where cancer cells are grown in Matrigel in modified six‐well plates. Oxygen is delivered to the cultures through a polydimethylsiloxane (PDMS) membrane at the bottom of the wells, with microfabricated PDMS pillars to control oxygen delivery. The plates receive 3% oxygen from below and 0% oxygen at the top surface of the media, providing a gradient of 3–0% oxygen. We compared growth and transcriptional profiles for cancer cells grown in Matrigel in the bioreactor, 3D cultures grown in 21% oxygen, and cells grown in a standard hypoxia chamber at 3% oxygen. Additionally, we compared gene expression of conventional two‐dimensional monolayer culture and 3D Matrigel culture in 21% oxygen. We conclude that controlled oxygen delivery may provide a more physiologically relevant 3D system.