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An endoplasmic reticulum stress regulator, Tmbim6 , modulates secretory stage of mice molar
Author(s) -
Aryal Yam Prasad,
Neupane Sanjiv,
Adhikari Nirpesh,
An ChangHyeon,
Ha JungHong,
Kwon TaeYub,
Yamamoto Hitoshi,
Jung JaeKwang,
Park EuiKyun,
Kim JiYoun,
Cho SungWon,
Sohn WernJoo,
Lee Youngkyun,
Chae HanJung,
Kim HyungRyong,
Kim JaeYoung
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28635
Subject(s) - odontoblast , ameloblast , endoplasmic reticulum , dentin , microbiology and biotechnology , unfolded protein response , molar , amelogenin , enamel paint , chemistry , biology , biochemistry , gene , dentistry , medicine , paleontology
Abstract To understand the role of endoplasmic reticulum (ER)‐stress in mice molar development, we studied Tmbim6 that antagonizes the unfolded protein response, using Tmbim6 knockout (KO) mice and in vitro organ cultivation with knocking down using small interfering RNA. During molar development, Tmbim6 is expressed in developing tooth at E14–E16, postnatal0 (PN0), and PN6. Mineral content in Tmbim6 KO enamel was reduced while dentin was slightly increased revealing ultrastructural changes in pattern formation of both enamel and dentin. Moreover, odontoblast differentiation was altered with increased Dspp expression at PN0 followed by altered AMELX localizations at PN5. These results were confirmed by in vitro organ cultivation and showed altered Bmp signaling, proliferation, and actin rearrangement in the presumptive ameloblast and odontoblasts that followed the altered expression of differentiation and ER stress‐related signaling molecules at E16.5. Overall, ER stress modulated by Tmbim6 would play important roles in patterned dental hard tissue formation in mice molar within a limited period of development.