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Brefeldin A impairs porcine oocyte meiotic maturation via interruption of organelle dynamics
Author(s) -
Cui Zhaokang,
Yu Lingzhu,
Shi Yang Xiayan,
Zhang Yu,
Shi Xiaoyan,
Li Yu,
Chen Qiuju,
Xiong Bo
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28611
Subject(s) - microbiology and biotechnology , brefeldin a , golgi apparatus , oocyte , endoplasmic reticulum , microinjection , cytoplasm , meiosis , biology , cyclin b1 , mitosis , oocyte activation , microtubule , chemistry , cell cycle , cyclin dependent kinase 1 , biochemistry , apoptosis , embryo , gene
Brefeldin A (BFA) is a lactone antibiotic synthesized from palmitic acid by several fungi that could block anterograde transport of proteins from endoplasmic reticulum to Golgi apparatus by reversible disruption of the Golgi complex. Previous investigations have shown that BFA induces the apoptosis of cancer cells in mitosis and impairs asymmetric spindle positioning in meiosis. Here, we document that exposure to BFA in porcine oocytes compromises the meiotic maturation via disrupting both nuclear and cytoplasmic maturation. We found that BFA exposure collapsed the cytoskeleton assembly by showing the aberrant spindle organization with misaligned chromosomes and defective actin dynamics. Furthermore, the distribution of both mitochondria and cortical granules (CGs), two important indexes of cytoplasmic maturation of oocytes, was disturbed following BFA exposure. We finally validated that the localization of ovastacin, a component of CGs that is essential for the postfertilization removal of sperm‐binding sites in the zona pellucida, was also perturbed in BFA‐exposed oocytes, which might weaken their fertilization capacity. Collectively, these findings indicate that Golgi‐mediated protein transport is indispensable for the porcine oocyte meiotic maturation.