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Combinatorial effects of radiofrequency hyperthermia and radiotherapy in the presence of magneto‐plasmonic nanoparticles on MCF‐7 breast cancer cells
Author(s) -
Hadi Fahimeh,
Tavakkol Shima,
Laurent Sophie,
Pirhajati Vahid,
Mahdavi Seied Rabi,
Neshastehriz Ali,
ShakeriZadeh Ali
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28599
Subject(s) - hyperthermia , mcf 7 , viability assay , apoptosis , mtt assay , cancer cell , chemistry , microbiology and biotechnology , cancer research , breast cancer , nanoparticle , iron oxide nanoparticles , cancer , biophysics , materials science , medicine , nanotechnology , biology , biochemistry , human breast
Abstract Here, the effects of combinatorial cancer therapy including radiotherapy (RT) and radiofrequency (RF) hyperthermia in the presence of gold‐coated iron oxide nanoparticles (Au@IONPs), as a thermo‐radio‐sensitizer, are reported. The level of cell death and the ratio of Bax/Bcl2 genes, involved in the pathway of apoptosis, were measured to evaluate the synergistic effect of Au@IONPs‐mediated RF hyperthermia and RT. MCF‐7 human breast adenocarcinoma cells were treated with different concentrations of Au@IONPs. After incubation with NPs, the cells were exposed to RF waves (13.56 MHz; 100 W; 15 min). At the same time, thermometry was performed with an infrared (IR) camera. Then, the cells were exposed to 6 MV X‐ray at various doses of 2 and 4 Gy. MTT (3‐[4,5‐dimethylthiazol‐2‐y1]‐2,5‐diphenyltetrazolium bromide) assay was performed to evaluate cell viability and quantitative real‐time polymerase chain reaction (qRT‐PCR) was used to determine the expression ratio of Bax/Bcl2. Cellular uptake of nanoparticles was confirmed qualitatively and quantitatively. The results obtained from MTT assay and qRT‐PCR studies showed that NPs and RF hyperthermia had no significant effect when applied separately, while their combination had synergistic effects on cell viability percentage and the level of apoptosis induction. A synergistic effect was also observed when the cancer cells were treated with a combination of NPs, RF hyperthermia, and RT. On the basis of the obtained results, it may be concluded that the use of magneto‐plasmonic NPs in the process of hyperthermia and RT of cancer holds a great promise to develop a new combinatorial cancer therapy strategy.