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miR‐548c‐5p inhibits colorectal cancer cell proliferation by targeting PGK1
Author(s) -
Ge Jianxin,
Li Jun,
Na Su,
Wang Pingping,
Zhao Guifeng,
Zhang Xiaoyan
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28525
Subject(s) - colorectal cancer , microrna , cancer research , carcinogenesis , suppressor , cell growth , peripheral blood mononuclear cell , cancer , pathogenesis , biology , gene , immunology , biochemistry , genetics , in vitro
Accumulating studies have implicated that microRNAs (miRNAs) are involved in the pathogenesis of colorectal cancer (CRC). However, the role of miR‐548c‐5p, a novel identified miRNA in malignancies, in colorectal carcinogenesis remains largely unknown. The present study is aimed to investigate the effect and molecular mechanism of miR‐548c‐5p in CRC by a sequence of cellular experiments. miR‐548c‐5p was significantly downregulated, whereas phosphoglycerate kinase 1 (PGK1), a key enzyme for glycolysis, was obviously upregulated in peripheral blood mononuclear cells and cancer tissues from patients with CRC. Besides, miR‐548c‐5p and PGK1 were negatively associated with each other. The luciferase reporter assay revealed that PGK1 was a targeted gene of miR‐548c‐5p. Moreover, the proliferation and generation of inflammatory cytokines (TNF‐α and IL‐6) were significantly inhibited in miR‐548c‐5p‐overexpressed SW480 CRC cells stimulated by lipopolysaccharide (LPS). Accordingly, miR‐548c‐5p may serve as a cancer suppressor in CRC by targeting PGK1.