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Protective effects of microRNA‐22‐3p against retinal pigment epithelial inflammatory damage by targeting NLRP3 inflammasome
Author(s) -
Hu Zizhong,
Lv Xuehua,
Chen Lu,
Gu Xunyi,
Qian Huiming,
Fransisca Silvia,
Zhang Zhengyu,
Liu Qinghuai,
Xie Ping
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28523
Subject(s) - inflammasome , microrna , downregulation and upregulation , messenger rna , retinal , microbiology and biotechnology , retinal pigment epithelium , in vivo , biology , inflammation , chemistry , immunology , gene , genetics , biochemistry
Abstract NLRP3, as a crucial inflammasome component, plays important roles in age‐related macular degeneration. Though some activators of NLRP3 have been studied, microRNAs (miRNAs) which potentially regulate NLRP3 messenger RNA (mRNA) have not been fully explored in retinal pigment epithelial (RPE) cells and retinopathy. In this study, by miRNA microarray proling and bioinformatic analysis, we identified that four miRNAs, miR‐4286, miR‐223‐3p, miR‐365a, miR‐22‐3p, may target NLRP3 mRNA in RPE inflammatory damage in vivo. Further, real‐time polymerase chain reaction verified that only miR‐22‐3p was significantly decreased, which was associated with NLRP3 upregulation in blue‐light‐induced retinopathy. Mechanistically, the dual‐fluorescent reporter suggested miR‐22‐3p directly binds NLRP3 mRNA. Moreover, overexpression of miR‐22‐3p could significantly reduce whereas inhibition miR‐22‐3p could increase the mRNA and protein expressions of NLRP3, Caspase‐1, and mature IL‐1β. Collectively, our results indicate that miR‐22‐3p plays a suppressive role in RPE damage by targeting NLRP3, which provides new insights into the future intervention to retinopathy.