Premium
microRNA‐329 suppresses epithelial‐to‐mesenchymal transition and lymph node metastasis in bile duct cancer by inhibiting laminin subunit beta 3
Author(s) -
Liao ChunHong,
Liu Yi,
Wu YiFei,
Zhu SiWei,
Cai RongYao,
Zhou Lei,
Yin XinMin
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28404
Subject(s) - epithelial–mesenchymal transition , metastasis , laminin , microrna , cancer research , protein subunit , duct (anatomy) , chemistry , cancer , biology , medicine , pathology , microbiology and biotechnology , gene , extracellular matrix , biochemistry
Bile duct cancer (BDC), also known as cholangiocarcinoma, is a highly desmoplastic cancer with a growth pattern characterized by periductal extension and infiltration. Studies have suggested that microRNAs (miRNAs) play an important role in BDC progression. Here we aim at investigating the effects of miR‐329 on BDC development, focusing especially on epithelial‐to‐mesenchymal transition (EMT) in vitro and lymph node metastasis in vivo. Expression microarrays associated with BDC tissues were collected and differentially expressed genes were analyzed, followed by miRNA target prediction and verification. The role miR‐329 played in BDC was examined using gain‐of‐function and loss‐of‐function methods. The expressions of miR‐329, laminin subunit beta 3 (LAMB3), and EMT markers, in addition to cell proliferation, migration, and invasion were evaluated. Furthermore, nude mice models of BDC were established to observe tumor growth and metastatic lymph nodes. The LAMB3 was identified as an upregulated gene based on the GSE77984 and GSE45001 microarray analysis. LAMB3 was also predicted and confirmed to be a target gene of miR‐329 by dual‐luciferase reporter assay. Through further cell experiments, the EMT process was reversed, cell proliferation, invasion, and migration were suppressed, when miR‐329 was upregulated. Furthermore, in vivo experiments exhibited that the overexpression of miR‐329 inhibited tumor growth and the number of metastatic lymph nodes. This study provides in vivo and in vitro evidence that miR‐329 inhibits BDC progression through translational repression of LAMB3. Therefore, the obtained results may aid as an experimental basis for improving prognosis of BDC.