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miRNA‐145‐5p induces apoptosis after ischemia‐reperfusion by targeting dual specificity phosphatase 6
Author(s) -
Wu Gang,
Tan Jiaying,
Li Junping,
Sun Xiaoli,
Du Lei,
Tao Sun
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28291
Subject(s) - apoptosis , phosphatase , microrna , ischemia , microbiology and biotechnology , dual specificity phosphatase , dual (grammatical number) , reperfusion injury , chemistry , biology , medicine , biochemistry , gene , phosphorylation , art , literature
Disorders mainly caused by ischemia‐reperfusion (I/R), including stroke and myocardial infarction, is linked to debilitating health conditions and death. Recent research indicates that microRNAs (miRNAs) mediate the process of ischemic pathology. This study investigated the effects of miR‐145‐5p in regulating myocardial ischemic injury. The I/R models were established in rat cardiomyocytes H9C2 and rats. Western blot analysis and quantitative polymerase chain reaction was performed to analyze protein expression. Annexin V‐FITC/PI staining was conducted to evaluate cell apoptosis. The application of miR‐145‐5p mimics and inhibitor revealed that miR‐145‐5p promoted apoptosis in cardiomyocytes. Furthermore, we found that miR‐145‐5p directly inhibited dual specificity phosphatase 6 (DUSP6) by luciferase reporter assay. The results indicated that DUSP6 was beneficial against I/R injury through inhibiting c‐Jun N‐terminal kinase pathways. In conclusion, the essential roles of miR‐145‐5p and DUSP6 in I/R provide a novel therapeutic target to develop future intervention strategies.