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A detailed image of rutin underlying intracellular signaling pathways in human SW480 colorectal cancer cells based on miRNAs‐lncRNAs‐mRNAs‐TFs interactions
Author(s) -
Nasri Nasrabadi Parinaz,
Zareian Somaye,
Nayeri Zahra,
Salmanipour Reza,
Parsafar Soha,
Gharib Ehsan,
Asadzadeh Aghdaei Hamid,
Zali Mohammad Reza
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28204
Subject(s) - rutin , microrna , biology , transcriptome , signal transduction , cell cycle , intracellular , microbiology and biotechnology , transcription factor , cell signaling , cell , biochemistry , gene expression , gene , antioxidant
Abstract Natural dietary ingredients like flavonoids are important for body improvement against diseases. The flavonol rutin is widely found in fruits and vegetables and shows significant anticancer properties. However, the underlined signaling pathways have not been elucidated yet. In this study, the impacts of various doses of rutin (400–700 mM/ml) have been examined on human colon cancer SW480 cells metabolism, cell cycle, and apoptosis. The transcriptome was analyzed by bioinformatics tools and the interactions between rutin modulated microRNAs (miRNAs), long noncoding RNAs (lncRNAs), messenger RNAs (mRNAs), and transcription factors (TFs) were built, filtered and enriched. A dose of 600 mM of rutin significantly decreased cells metabolic activity, halved the population and arrested the cell cycle at the sub‐G1 phase. The enrichment analysis of miRNAs‐lncRNAs‐mRNAs‐TFs network showed that these effects were mediated through alteration of glucose, lipid, and protein metabolism, modulating endoplasmic reticulum stress responses, negative regulation of cell cycle process, and inducing the extrinsic and intrinsic apoptotic signaling pathways. Additionally, the key parent nodes of each annotation were illustrated. These findings create a detailed image of rutin underlying intracellular signaling pathways in CRC and also help us to better understand the role of dietary natural compounds in cancer treatment.