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Improved cellular response on functionalized polypyrrole interfaces
Author(s) -
Naghavi Alhosseini Sanaz,
Moztarzadeh Fathollah,
Karkhaneh Akbar,
Dodel Masumeh,
Khalili Mahsa,
Eslami Arshaghi Tarlan,
Elahirad Elnaz,
Mozafari Masoud
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28173
Subject(s) - polypyrrole , gelatin , biocompatibility , materials science , surface modification , neural tissue engineering , tissue engineering , electrospinning , nanofiber , polymerization , nanotechnology , chemistry , biomedical engineering , biophysics , chemical engineering , polymer , organic chemistry , composite material , medicine , engineering , metallurgy , biology
Neuroregeneration strategies involve multiple factors to stimulate nerve regeneration. Neural support with chemical and physical cues to optimize neural growth and replacing the lesion neuron and axons are crucial for designing neural scaffolds, which is a promising treatment approach. In this study, polypyrrole polymerization and its functionalization at the interface developed by glycine and gelatin for further optimization of cellular response. Nanofibrous scaffolds were fabricated by electrospinning of polyvinyl alcohol and chitosan solutions. The electrospun scaffolds were polymerized on the surface by pyrrole monomers to form an electroactive interface for further applications in neural tissue engineering. The polymerized polypyrrole showed a positive zeta potential value of 57.5 ± 5.46 mV. The in vitro and in vivo biocompatibility of the glycine and gelatin‐functionalized polypyrrole‐coated scaffolds were evaluated. No inflammatory cells were observed for the implanted scaffolds. Further, DAPI nucleus staining showed a superior cell attachment on the gelatin‐functionalized polypyrrole‐coated scaffolds. The topography and tuned positively charged polypyrrole interface with gelatin functionalization is expected to be particularly efficient physical and chemical simultaneous factors for promoting neural cell adhesion.