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Novel cellular mechanism that mediates the collecting duct formation during postnatal renal development
Author(s) -
Guaytima Edith,
Brandán Yamila Romina,
Favale Nicolás Octavio,
SterinSpeziale Norma B.,
Márquez María Gabriela
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.28016
Subject(s) - mesenchymal stem cell , microbiology and biotechnology , kidney development , biology , embryonic stem cell , phenotype , kidney , duct (anatomy) , embryogenesis , in vivo , cellular differentiation , anatomy , embryo , endocrinology , gene , genetics
Abstract We have previously demonstrated that kidney embryonic structures are present in rats, and are still developing until postnatal Day 20. Consequently, at postnatal Day 10, the rat renal papilla contains newly formed collecting duct (CD) cells and others in a more mature stage. Performing primary cultures, combined with immunocytochemical and time‐lapse analysis, we investigate the cellular mechanisms that mediate the postnatal CD formation. CD cells acquired a greater degree of differentiation, as we observed that they gradually lose the ability to bind BSL‐I lectin, and acquire the capacity to bind Dolichos biflorus . Because CD cells retain the same behavior in culture than in vivo, and by using DBA and BSL‐I as markers of cellular lineage besides specific markers of epithelial/mesenchymal phenotype, the experimental results strongly suggest the existence of mesenchymal cell insertion into the epithelial CD sheet. We propose such a mechanism as an alternative strategy for CD growing and development.