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Sprouty1 regulates neuritogenesis and survival of cortical neurons
Author(s) -
Gu Xi,
Su Xiaohong,
Jia Chunhong,
Lin Lifang,
Liu Shuhu,
Zhang Peidong,
Wang Xuemin,
Jiang Xiaodan
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.27949
Subject(s) - neurite , biology , microbiology and biotechnology , receptor tyrosine kinase , neurotrophin , neurotrophic factors , neural stem cell , glutamate receptor , cellular differentiation , fibroblast growth factor , brain derived neurotrophic factor , neuroscience , stem cell , signal transduction , receptor , gene , biochemistry , in vitro
In multicellular organisms, receptor tyrosine kinases (RTKs) control a variety of cellular processes, including cell proliferation, differentiation, migration, and survival. Sprouty (SPRY) proteins represent an important class of ligand‐inducible inhibitors of RTK‐dependent signaling pathways. Here, we investigated the role of SPRY1 in cells of the central nervous system (CNS). Expression of SPRY1 was substantially higher in neural stem cells than in cortical neurons and was increased during neuronal differentiation of cortical neurons. We found that SPRY1 was a direct target gene of the CNS‐specific microRNA, miR‐124 and miR‐132 . In primary cultures of cortical neurons, the neurotrophic factors brain‐derived neurotrophic factor (BDNF) and Basic fibroblast growth factor (FGF2) downregulated SPRY1 expression to positively regulate their own functions. In immature cortical neurons and mouse N 2 A cells, we found that overexpression of SPRY1 inhibited neurite development, whereas knockdown of SPRY1 expression promoted neurite development. In mature neurons, overexpression of SPRY1 inhibited the prosurvival effects of both BDNF and FGF2 on glutamate‐mediated neuronal cell death. SPRY1 was also upregulated upon glutamate treatment in mature neurons and partially contributed to the cytotoxic effect of glutamate. Together, our results indicate that SPRY1 contributes to the regulation of CNS functions by influencing both neuronal differentiation under normal physiological processes and neuronal survival under pathological conditions.