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Long noncoding RNA GACAT3 promotes glioma progression by sponging miR‐135a
Author(s) -
Wang Jing,
Zhang Ming,
Lu Weifeng
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.27946
Subject(s) - competing endogenous rna , glioma , long non coding rna , carcinogenesis , microrna , cancer research , downregulation and upregulation , biology , rna , cancer , gene , genetics
The long noncoding RNA (lncRNA) gastric cancer associated transcript 3 (GACAT3) has been reported to play important roles in human tumorigenesis. However, its expression pattern, functions, and underlying mechanism in glioma remain unclear. In the present study, we showed that GACAT3 is upregulated in glioma tissues and cell lines. Through online databases, luciferase reporter assays and RNA immunoprecipitation (RIP) assays, we determined that GACAT3 acts as a competing endogenous RNA (ceRNA) for microRNA (miR)‐135a, which was downregulated and performed as a tumor inhibitor in glioma. Further, nicotinamide phosphoribosyl transferase (NAMPT) was confirmed as a target gene of miR‐135a by a series of gain‐ and loss‐of‐function assays. Overall, the present study was the first to show that GACAT3 regulates the expression of NAMPT to promote glioma progression by sponging miR‐135a. These findings provide a promising therapy strategy for glioma.