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Effects of microRNA‐513b on cell proliferation, apoptosis, invasion, and migration by targeting HMGB3 through regulation of mTOR signaling pathway in non‐small‐cell lung cancer
Author(s) -
Wang Jiying,
Sheng Zhaoying,
Cai Yong
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.27921
Subject(s) - apoptosis , flow cytometry , cell growth , biology , microrna , cancer research , pi3k/akt/mtor pathway , cell , microbiology and biotechnology , gene , biochemistry , genetics
Abstract This study aimed to explore the underlying mechanism of miR‐513b and HMGB3 in regulating non‐small‐cell lung cancer (NSCLC). NSCLC tumor, adjacent tissues, and cell lines were extracted, and the expression of miR‐513b and HMGB3 were determined by quantitative real‐time polymerase chain reaction (RT‐qPCR) and western blot analysis. Then, miR‐513b was overexpressed in NSCLC cell, and the proliferation, migration, invasion, and apoptosis of cells were determined by 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT), wound healing, transwell, and flow cytometry, respectively. Regulatory relationship between miR‐513b and HMGB3 was determined using luciferase activity reporter assay. Lastly, HMGB3 and/or miR‐513b were overexpressed in NSCLC cells, and the proliferation, migration, invasion, and apoptosis of cells were determined. Compared with the controls, the expression of miR‐513b was significantly downregulated in the NSCLC tissues and cells lines by RT‐qPCR ( p < 0.05). However, the expression of HMGB3 was significantly downregulated at both messenger RNA and protein levels ( p < 0.05). Overexpression of miR‐513b could significantly inhibit the proliferation, invasion, migration, and promote apoptosis of NSCLC cells ( p < 0.05). HMGB3 was a target of miR‐513b, and overexpression of HMGB3 could obviously reverse the effect of miR‐513 on the proliferation, invasion, migration, and apoptosis of NSCLC cells ( p < 0.05). The present results could suggest miR‐513b was downregulated in NSCLC and could regulate the proliferation, invasion, migration, and apoptosis of NSCLC cells via HMGB3.