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Influence of colonic dialysis using Gubenxiezhuo on the distribution of gut microflora in uremia rats
Author(s) -
He Haidong,
Hu Ping,
Tang Yuyan,
Xu Xudong
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.27845
Subject(s) - uremia , downregulation and upregulation , dialysis , gut flora , medicine , endocrinology , microbiology and biotechnology , biology , immunology , biochemistry , gene
Objective This study aimed to explore the underlying function of Gubenxiezhuo dialysis on the distribution of gut microflora uremia. Methods A uremia rat model was constructed, and the morphology of renal tissue was determined using the hematoxylin‐eosin (H&E) staining. Moreover, the blood samples were collected and the expression of IL‐1β, IL‐6, and CRP was determined using enzyme‐linked immunosorbent assay. Following these experiments, the gut tissues of rats were collected and the distribution of gut microbiota was explored using real‐time PCR. Results Compared with the control group, inflammatory infiltration, apoptosis, and bleeding were significantly upregulated in kidney of uremia rats, and Gubenxiezhuo dialysis could obviously ameliorate these changes. Expression of IL‐1β, IL‐6, and CRP were significantly elevated in uremic rats and Gubenxiezhuo could significantly attenuate these elevations ( p < 0.01). In addition, Gubenxiezhuo dialysis also could attenuate the upregulations of Acinetobacter , Bacillus cereus , Proteus vulgaris , Shigella flexneri , and Escherichia coli , and the downregulation of Bifidobacterium , Lactobacillus , and Helicobacter in the uremia rats ( p < 0.05). Conclusion Gubenxiezhuo dialysis could significantly ameliorate the inflammatory to modulate the distribution of gut microbiota in uremia.