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Intracellular delivery of nucleic acid by cell‐permeable hPP10 peptide
Author(s) -
Ding Yi,
Zhao Xueli,
Geng Jingping,
Guo Xiangli,
Ma Jielan,
Wang Hu,
Liu Changbai
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.27826
Subject(s) - nucleic acid , transfection , gene delivery , rna , transduction (biophysics) , small interfering rna , microbiology and biotechnology , green fluorescent protein , dna , biology , plasmid , intracellular , reporter gene , chemistry , gene , biochemistry , gene expression
Although gene therapy offers hope against incurable diseases, nonreplicating transduction vectors remain lacking. We have previously characterized a cell‐penetrating peptide hPP10 for the delivery of various cargoes; however, whether hPP10 can mediate nucleic acid delivery is still unknown. Here, examining via different ways, we demonstrate that hPP10 stably complexes with plasmid DNA (pDNA) and safely mediates nucleic acid transfection. hPP10 can mediate GFP‐, dsRed‐, and luciferase‐expressing plasmids into cells with nearly the same efficiency as commercial transfection reagents Turbofectin or Lipofect. Furthermore, hPP10 can mediate Cre fusion protein delivery and pDNA transfection simultaneously in the Cre/loxp system in vitro. In addition, hPP10 fused with an RNA‐binding domain can mediate delivery of small interfering RNA into cells to silence the reporter gene expression. Collectively, our results suggest that hPP10 is an option for nucleic acid delivery with efficiencies similar to that of commercial reagents.