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Overexpression of GAS5 inhibits abnormal activation of Wnt/β‐catenin signaling pathway in myocardial tissues of rats with coronary artery disease
Author(s) -
Li Xuexiang,
Hou Linlin,
Cheng Ziping,
Zhou Shu,
Qi Jinwei,
Cheng Jinglin
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.27792
Subject(s) - gas5 , wnt signaling pathway , coronary artery disease , downregulation and upregulation , oxidative stress , hyperlipidemia , medicine , catenin , apoptosis , cancer research , signal transduction , long non coding rna , cad , endocrinology , biology , microbiology and biotechnology , gene , diabetes mellitus , biochemistry
Abstract Objective The aim of this study is to investigate the clinical value of long noncoding RNA growth arrest‐specific transcript 5 (LncRNA GAS5) in the diagnosis of coronary artery disease (CAD) and its protective effect on myocardial injury in rats with CAD. Methods Patients with CAD and healthy controls were selected to measure the expression of GAS5, and further to perform the correlation analysis and ROC curve. In addition, the rat models of CAD were also established to observe the effect of GAS5 on hyperlipidemia, myocardial injury, cardiomyocyte apoptosis, oxidative stress, and inflammatory injury of rats with CAD, and the effect of the Wnt/β‐catenin signaling pathway was also determined. Results Overexpression of GAS5 in CAD rats determines improvement of hyperlipidemia, attenuation of myocardial injury, inhibition of cardiomyocyte apoptosis, oxidative stress, inflammatory injury, and abnormal activation of the Wnt/β‐catenin signaling pathway in myocardial tissues. Conclusion Our study demonstrates that downregulation of GAS5 is found in CAD, and overexpression of GAS5 inhibits abnormal activation of the Wnt/β‐catenin signaling pathway in myocardial tissues of CAD rats.