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LncRNA miR143HG suppresses bladder cancer development through inactivating Wnt/β‐catenin pathway by modulating miR‐1275/AXIN2 axis
Author(s) -
Xie Hui,
Huang Hang,
Huang Weiping,
Xie Zhiyue,
Yang Yu,
Wang Feng
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.27764
Subject(s) - axin2 , wnt signaling pathway , gene knockdown , ectopic expression , cancer research , biology , catenin , cell growth , in vivo , microbiology and biotechnology , cell culture , signal transduction , genetics
Although increasing long noncoding RNAs (lncRNAs) have been identified by high‐throughput sequencing, their functions in human cancer remain largely unknown. The function of lncRNA miR143HG has not been explored before. In the present study, we found that miR143HG expression was significantly downregulated in bladder cancer tissues (BCa) compared with normal tissues. We showed that miR143HG high expression was associated with a high survival rate in BCa patients. Gain‐of‐function assays demonstrated that miR143HG overexpression suppressed the proliferation, arrested cell cycle progression, and attenuated migration and invasion of BCa cells in vitro. In vivo assay illustrated that ectopic expression of miR143HG inhibited BCa growth in vivo. Mechanistically, miR143HG was identified to inhibit the level of miR‐1275, whereas miR‐1275 directly targeted AXIN2, a negative regulator of the Wnt/β‐catenin pathway. Restoration of miR‐1275 or knockdown of AXIN2 significantly rescued the proliferation, migration, and invasion abilities of BCa cells. In summary, our findings demonstrated that miR143HG/miR‐1275/AXIN2 axis regulates BCa development by modulating the Wnt/β‐catenin pathway.