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Sulfonyl(thio)urea derivative induction of insulin secretion is mediated by potassium, calcium, and sodium channel signal transduction
Author(s) -
Sulis Paola Miranda,
Dambrós Betina Fernanda,
Mascarello Alessandra,
dos Santos Adair Roberto Soares,
Yunes Rosendo Augusto,
Nunes Ricardo José,
Frederico Marisa Jádna Silva,
Barreto Silva Fátima Regina Mena
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.27680
Subject(s) - urea , chemistry , potassium channel , potassium , secretion , thio , signal transduction , calcium , sodium , sulfonyl , calcium channel , endocrinology , biochemistry , medicine , microbiology and biotechnology , biology , stereochemistry , alkyl , organic chemistry
Aim To investigate the mechanism of action of sulfonyl(thio)urea derivative (SD) on glycemia and on insulin secretion in pancreatic islets. Methods Wistar rats were divided into hyperglycemic control group, rats received 4 g/kg body weight glucose plus sitagliptin 10 mg/kg (p.o.); hyperglycemic plus SD 10 mg/kg (p.o.); hyperglycemic plus SD plus sitagliptin. Blood was collected before glucose overloading (zero time), and at 15, 30, 60, and 180 min after glucose, from the afore mentioned groups for glycemia and glucagon‐like peptide 1 (GLP‐1) measurements and intestinal disaccharidases activity. Pancreatic islets were isolated for the calcium influx and insulin secretion in in vitro studies. Results SD reduced glycemia and increased GLP‐1 secretion, while inhibited sucrase and lactase activity. This SD (1.0 and 10.0 µM) stimulated calcium influx in a similar percentile to that of glibenclamide, and in a nonsynergic manner. In addition, the trigger effect of SD on calcium influx was through the K + ‐ATP‐dependent channels, and partially by activating voltage‐dependent K + channels and voltage‐dependent calcium channels. Furthermore, SD‐stimulated Na + and Ca 2+ entry, induced by the transient receptor potential ankyrin 1 and by modulation of Na + /Ca 2+ exchange. The activation of these pathways by SD culminated in in vitro insulin secretion, reinforcing the critical role of K + ‐ATP channels in the secretagogue effect of SD. Conclusions SD diminish glycemia by inducing GLP‐1 secretion and inhibiting disaccharidases. To our knowledge, this is the first report of an insulin secretagogue effect of SD that is mediated by potassium and calcium, as well as sodium, signal transduction.