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Let‐7g‐5p regulates mouse mammary cells differentiation and function by targeting PRKCA
Author(s) -
Tian Lei,
Li Ye,
Wang Chunmei,
Li Qingzhang
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.27676
Subject(s) - biology , microrna , ectopic expression , mammary gland , microbiology and biotechnology , untranslated region , reporter gene , gene expression , regulation of gene expression , messenger rna , gene , transfection , cellular differentiation , genetics , cancer , breast cancer
MicroRNAs (miRNAs) are closely related with the posttranscriptional regulation of gene expression. Our past study showed that let‐7g‐5p decreased during pregnancy and lactation in mouse mammary gland, what suggested the prospective regulating role of let‐7g‐5p in mammary epithelial cells. In this study, to unravel the role of let‐7g‐5p, we found PRKCA (PKC‐alpha, PKC‐α) might serve as potential targets of let‐7g‐5p by bioinformatics. Then let‐7g‐5p was knocked down by an antisense oligonucleotide in mouse primary mammary epithelial cells. As predicted, PRKCA gene expression and mammary epithelial cells growth were increased by anti‐let‐7g‐5p regulation in vitro. By using reporter constructs, it showed that the let‐7g‐5p could direct binding with 3′‐the untranslated regions (3′‐UTR) of PRKCA mRNA. Furthermore, ectopic overexpression of let‐7g‐5p in vivo reduced PRKCA and β‐casein protein expression as well as inhibited mammary gland growth. These results suggested that let‐7g‐5p could inhibit the mammary epithelial cells differentiation and β‐casein protein synthesis and expression through suppression of PRKCA on the cycle of mammary cell differentiation and development and that let‐7g‐5p may be a novel important regulated target in mammary cells function.