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Estradiol‐17β stimulates H 2 S biosynthesis by ER‐dependent CBS and CSE transcription in uterine artery smooth muscle cells in vitro
Author(s) -
Lechuga Thomas J.,
Bilg Amanpreet K.,
Patel Bansari A.,
Nguyen Nicole A.,
Qi Qianrong,
Chen Dongbao
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.27606
Subject(s) - cystathionine beta synthase , estrogen receptor , agonist , messenger rna , in vitro , in vivo , transcription (linguistics) , endogeny , receptor , estrogen , medicine , endocrinology , biology , chemistry , microbiology and biotechnology , enzyme , biochemistry , gene , linguistics , philosophy , cancer , cysteine , breast cancer
Endogenous hydrogen sulfide (H 2 S), synthesized by cystathionine β‐synthase (CBS) and cystathionine γ‐lyase (CSE), is a potent vasodilator that can be stimulated by estradiol‐17β (E 2 β) in uterine artery (UA) smooth muscle (UASMC) in vivo; however, the underlying mechanisms are unknown. This study tested a hypothesis that E 2 β stimulates H 2 S biosynthesis by upregulating CBS expression via specific estrogen receptor (ER). Treatment with E 2 β stimulated time‐ and concentration‐ dependent CBS and CSE messenger RNA (mRNA) and protein expressions, and H 2 S production in cultured primary UASMC isolated from late pregnant ewes, which were blocked by ICI 182,780. Treatment with specific ERα or ERβ agonist mimicked these E 2 β‐stimulated responses, which were blocked by specific ERα or ERβ antagonist. Moreover, E 2 β activated both CBS and CSE promoters and ICI 182,780 blocked the E 2 β‐stimulated responses. Thus, E 2 β stimulates H 2 S production by upregulating CBS and CSE expression via specific ER‐dependent transcription in UASMC in vitro.