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Oleuropein inhibits migration ability through suppression of epithelial‐mesenchymal transition and synergistically enhances doxorubicin‐mediated apoptosis in MCF‐7 cells
Author(s) -
Choupani Jalal,
Alivand Mohammad R.,
M. Derakhshan Sima,
Zaeifizadeh Mohammad,
S. Khaniani Mahmoud
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.27586
Subject(s) - mcf 7 , apoptosis , epithelial–mesenchymal transition , doxorubicin , chemistry , microbiology and biotechnology , mesenchymal stem cell , transition (genetics) , cancer research , biology , medicine , biochemistry , chemotherapy , cancer cell , gene , human breast , cancer
Distinct metastasis is one of the main causes of breast cancer (BC)‐related mortality and epithelial‐mesenchymal transition (EMT) is a primary step in metastasis dissemination. On the other hand, doxorubicin (DOX) is an effective chemotherapeutic agent against BC; unfortunately, its clinical use is limited by dose‐dependent side effects. Therefore, extensive efforts have been dedicated to suppressing metastasis of BC and also to overcome DOX side effects together with keeping its antitumor efficacy. Studies supported the role of oleuropein (OLEU) in reducing DOX‐induced side effects besides its antitumor actions. In this study, the antimigratory effect of OLEU was assessed and real‐time PCR (RT‐PCR) was used to detect OLEU effect on the expression level of EMT markers, in MCF‐7 cells. The cytotoxic effect of OLEU and DOX was assessed by MTT assay, whereas the ratio of apoptosis was investigated by flow cytometry. The results showed that migration ability of MCF‐7 cells remarkably decreased in OLEU treated group and RT‐PCR results showed that OLEU may exert its antimigratory action by suppressing EMT through downregulation of sirtuin1 (SIRT1). Also, the results indicated that both OLEU and DOX were cytotoxic to MCF‐7 cells, whereas DOX‐OLEU cotreatment led to additive cytotoxicity and apoptosis rate. This study provides evidence regarding the suppressive role of OLEU on MCF‐7 cells migration ability through suppression of EMT, and for the first time, it was proposed that SIRT1 downregulation can be involved in the OLEU antimigratory effect. Also, the findings demonstrated that OLEU can reduce DOX‐induced side effects by reducing its effective dose.