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EA15, MIR22, LINC00472 as diagnostic markers for diabetic kidney disease
Author(s) -
Wang YanZhe,
Zhu DingYu,
Xie XinMiao,
Ding Miao,
Wang YongLan,
Sun LinLin,
Zhang Nan,
Shen E.,
Wang XiaoXia
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.27539
Subject(s) - gene , biology , disease , gene expression , immune system , rna , messenger rna , long non coding rna , interferon , computational biology , function (biology) , cancer research , bioinformatics , genetics , medicine
This study aimed to investigate the molecular mechanisms of diabetic kidney disease (DKD) and to explore new potential therapeutic strategies and biomarkers for DKD. First we analyzed the differentially expressed changes between patients with DKD and the control group using the chip data in Gene Expression Omnibus (GEO) database. Then the gene chip was subjected to be annotated again, so as to screen long noncoding RNAs (lncRNAs) and study expression differences of these lncRNAs in DKD and controlled samples. At last, the function of the differential lncRNAs was analyzed. A total of 252 lncRNAs were identified, and 14 were differentially expressed. In addition, there were 1,629 differentially expressed messenger RNAs (mRNAs) genes, and proliferation and apoptosis adapter protein 15 ( PEA15 ), MIR22 , and long intergenic nonprotein coding RNA 472 ( LINC00472 ) were significantly differentially expressed in DKD samples. Through functional analysis of the encoding genes coexpressed by the three lncRNAs, we found these genes were mainly enriched in type 1 diabetes and autoimmune thyroid disease pathways, whereas in Gene Ontology (GO) function classification, they were also mainly enriched in the immune response, type I interferon signaling pathways, interferon‐γ mediated signaling pathways, and so forth. To summary, we identified EA15 , MIR22 , and LINC00472 may serve as the potential diagnostic markers of DKD.