Premium
Identification of intracellular peptides associated with thermogenesis in human brown adipocytes
Author(s) -
Li Yun,
Wang Xing,
Wang Fei,
You Lianghui,
Xu Pengfei,
Cao Yan,
Chen Ling,
Wen Juan,
Guo Xirong,
Cui Xianwei,
Ji Chenbo
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.27465
Subject(s) - biology , thermogenesis , brown adipose tissue , intracellular , microbiology and biotechnology , kegg , thermogenin , receptor , biochemistry , adipose tissue , transcriptome , gene , gene expression
Objectives Currently, brown adipose tissue (BAT) is a therapeutic target in obesity and diabetes, but the mechanism of BAT activation remains unclear. Because increasing emphasis has been placed on the role of intracellular peptides in biological processes, we conducted a study to gain insight into the mechanism of BAT activation by using a peptidomic approach and then attempted to identify peptides that are capable of activating BAT. Methods In the present study, we generated the peptidomic profile of the intracellular peptides in brown adipocytes treated with forskolin (FSK) using a peptidomic approach. Then, the differentially expressed peptides were evaluated via Gene Ontology (GO) enrichment, KEGG pathway, and protein–protein interaction (PPI) network analysis. Finally, we selected candidate peptides for further validation via assessing the expression levels of UCP‐1 and PGC‐1α in brown adipocytes exposed to the peptides. Results A total of 4,370 peptides were identified, of which 951 were upregulated and 379 were downregulated after FSK treatment. Bioinformatic analysis demonstrated that the ECM‐receptor interaction GO term was the most enriched and that collagen alpha‐related proteins exhibited the highest degree of PPI. Four peptides separately derived from TSC22 domain family protein 1 (T22D1), bromodomain and WD repeat‐containing protein 1 (BRWD1), protein piccolo (PCLO), and collagen alpha‐1 (III) chain (CO3A1) increased the expression levels of UCP‐1 and PGC‐1α. Conclusions ECM‐receptor interaction may play an important role in the process of FSK‐stimulated BAT activation, and the pT22D1tide, pBRWD1tide, pPCLOtide, and pCO3A1tide peptides potentially promote BAT thermogenesis.