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Snake venoms promote stress‐induced senescence in human fibroblasts
Author(s) -
Lewinska Anna,
Bocian Aleksandra,
Petrilla Vladimir,
AdamczykGrochala Jagoda,
Szymura Karolina,
Hendzel Wiktoria,
Kaleniuk Edyta,
Hus Konrad K.,
Petrillova Monika,
Wnuk Maciej
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.27382
Subject(s) - naja , biology , snake venom , venom , fibroblast , senescence , toxicity , oxidative stress , toxicology , microbiology and biotechnology , cell culture , biochemistry , chemistry , genetics , organic chemistry
Snake venoms are widely studied in terms of their systemic toxicity and proteolytic, hemotoxic, neurotoxic, and cytotoxic activities. However, little is known about snake‐venom‐mediated effects when used at low, noncytotoxic concentrations. In the current study, two human fibroblast cell lines of different origin, namely WI‐38 fetal lung fibroblasts and BJ foreskin fibroblasts were used to investigate snake‐venom‐induced adaptive response at a relatively noncytotoxic concentration (0.01 µg/ml). The venoms of Indochinese spitting cobra ( Naja siamensis ), western green mamba ( Dendroaspis viridis ), forest cobra ( Naja melanoleuca ), and southern copperhead ( Agkistrodon contortrix ) were considered. Snake venoms promoted FOXO3a‐mediated oxidative stress response and to a lesser extent DNA damage response, which lead to changes in cell cycle regulators both at messenger RNA and protein levels, limited cell proliferation and migration, and induced cellular senescence. Taken together, we have shown for the first time that selected snake venoms may also exert adverse effects when used at relatively noncytotoxic concentrations.