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HDAC8 regulates canonical Wnt pathway to promote differentiation in skeletal muscles
Author(s) -
Ferrari Luca,
Bragato Cinzia,
Brioschi Loredana,
Spreafico Marco,
Esposito Simona,
Pezzotta Alex,
Pizzetti Fabrizio,
MorenoFortuny Artal,
Bellipanni Gianfranco,
Giordano Antonio,
Riva Paola,
Frabetti Flavia,
Viani Paola,
Cossu Giulio,
Mora Marina,
Marozzi Anna,
Pistocchi Anna
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.27341
Subject(s) - hdac8 , c2c12 , skeletal muscle , wnt signaling pathway , histone deacetylase , biology , microbiology and biotechnology , myocyte , histone , endocrinology , signal transduction , genetics , myogenesis , gene
Histone deacetylase 8 (HDAC8) is a class 1 histone deacetylase and a member of the cohesin complex. HDAC8 is expressed in smooth muscles, but its expression in skeletal muscle has not been described. We have shown for the first time that HDAC8 is expressed in human and zebrafish skeletal muscles. Using RD/12 and RD/18 rhabdomyosarcoma cells with low and high differentiation potency, respectively, we highlighted a specific correlation with HDAC8 expression and an advanced stage of muscle differentiation. We inhibited HDAC8 activity through a specific PCI‐34051 inhibitor in murine C2C12 myoblasts and zebrafish embryos, and we observed skeletal muscles differentiation impairment. We also found a positive regulation of the canonical Wnt signaling by HDAC8 that might explain muscle differentiation defects. These findings suggest a novel mechanism through which HDAC8 expression, in a specific time window of skeletal muscle development, positively regulates canonical Wnt pathway that is necessary for muscle differentiation.