z-logo
Premium
Nanos2 promotes differentiation of male germ cells basing on the negative regulation of Foxd3 and the treatment of 5‐Azadc and TSA
Author(s) -
Zhang Wenhui,
Bi Yulin,
Wang Yingjie,
Wang Man,
Li Dong,
Cheng Shaoze,
Jin Jing,
Li Tingting,
Li Bichun,
Zhang Yani
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.27139
Subject(s) - biology , epigenetics , transcription factor , dna methylation , transcription (linguistics) , gene , regulator , methylation , histone , regulation of gene expression , promoter , microbiology and biotechnology , genetics , gene expression , linguistics , philosophy
The transcription factor positioning in promoter regions relate to gene regulation, and the level of DNA methylation and histone acetylation also impact the promoter activity. In this study, we tested and verified the core promoter region and key transcription factor of Nanos2 which is a male‐critical gene in the differentiation of embryonic stem cells to male germ cells, meanwhile, epigenetic effects by mean of 5‐Aza‐2′‐deoxycytidine (5‐Azadc) and Trichostin A (TSA) on the activity of Nanos2 promoter were detected. The results reveal that key transcription factor Foxd3 is a negative regulator of Nanos2 , which suggests that loss‐of‐function of Foxd3 causes strong expression of Nanos2 responsive to large amounts of primordial germ cells and spermatogonial stem cells,whereas its overexpression causes the opposite effect. Furthermore, both 5‐Azadc and TSA can provoke responses of Nanos2 , but the combination effect of the two is better.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here