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Histone methyltransferase SETD2 is required for meiotic maturation in mouse oocyte
Author(s) -
Li Chunling,
Diao Feiyang,
Qiu Danhong,
Jiang Manxi,
Li Xiaoyan,
Han Longsen,
Li Ling,
Hou Xiaojing,
Ge Juan,
Ou Xianghong,
Liu Jiayin,
Wang Qiang
Publication year - 2019
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.26836
Subject(s) - meiosis , oocyte , microbiology and biotechnology , polar body , kinetochore , biology , chromosome segregation , histone methyltransferase , spindle checkpoint , aneuploidy , genetics , histone , chromosome , embryo , dna , gene
SET‐domain‐containing 2 (SETD2), a member of the histone lysine methyltransferase family, has been reported to be involved in multiple biological processes. However, the function of SETD2 during oocyte maturation has not been addressed. In this study, we find that mouse oocytes are incapable of progressing through meiosis completely once SETD2 is specifically depleted. These oocytes present an abnormal spindle morphology and deficient chromosome movement, with disrupted kinetochore–microtubule attachments, consequently producing aneuploidy eggs. In line with this, the BubR1 signal is markedly elevated in metaphase kinetochores of oocytes with SETD2 depletion, indicative of the activation of spindle assembly checkpoint. In addition, we note that loss of SETD2 results in a drastic decrease in the trimethylation level of H3K36 in oocytes. Collectively, our data demonstrate that SETD2 is required for oocyte maturation and indicate a novel mechanism controlling the meiotic apparatus.