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Crosstalk between adipose stem cells and tendon cells reveals a temporal regulation of tenogenesis by matrix deposition and remodeling
Author(s) -
CostaAlmeida Raquel,
Calejo Isabel,
Reis Rui L.,
Gomes Manuela E.
Publication year - 2018
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.26363
Subject(s) - microbiology and biotechnology , stem cell , tendon , extracellular matrix , crosstalk , mesenchymal stem cell , mmp1 , tissue engineering , adipose tissue , chemistry , anatomy , biology , biomedical engineering , medicine , gene expression , gene , biochemistry , physics , optics
Tendon injuries constitute an unmet clinical challenge owing to the limited intrinsic regenerative ability of this tissue. Cell‐based therapies aim at improving tendon healing through the delicate orchestration of tissue rebuilding and regain of function. Hence, human adipose‐derived stem cells (hASCs) have been proposed as a promising cell source for boosting tendon regeneration. In this work, we investigated the influence of hASCs on native human tendon‐derived cells (hTDCs) through the establishment of a direct contact co‐culture system. Results demonstrated that direct interactions between these cell types resulted in controlled proliferation and spontaneous cell elongation. ECM‐related genes, particularly COL1A1 and TNC , and genes involved in ECM remodeling, such as MMP1 , MMP2 , MMP3 , and TIMP1 , were expressed in co‐culture in a temporally regulated manner. In addition, deposition of collagen type I was accelerated in co‐culture systems and favored over the production of collagen type III, resulting in an enhanced COL1/COL3 ratio as soon as 7 days. In conclusion, hASCs seem to be good candidates in modulating the behavior of native tendon cells, particularly through a balanced process of ECM synthesis and degradation.