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Photobiomodulation of mesenchymal stem cells encapsulated in an injectable rhBMP4‐loaded hydrogel directs hard tissue bioengineering
Author(s) -
Diniz Ivana M. A.,
Carreira Ana C. O.,
Sipert Carla R.,
Uehara Cindi M.,
Moreira Maria S. N.,
Freire Laila,
Pelissari Cibele,
Kossugue Patrícia M.,
de Araújo Daniele R.,
Sogayar Mari C.,
Marques Márcia M.
Publication year - 2018
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.26309
Subject(s) - mesenchymal stem cell , in vivo , tissue engineering , stem cell , microbiology and biotechnology , chemistry , regeneration (biology) , cell , poloxamer , biomedical engineering , cancer research , biology , medicine , biochemistry , organic chemistry , copolymer , polymer
Photobiomodulation (PBM) therapy displays relevant properties for tissue healing and regeneration, which may be of interest for the tissue engineering field. Here, we show that PBM is able to improve cell survival and to interact with recombinant human Bone Morphogenetic Protein 4 (rhBMP4) to direct and accelerate odonto/osteogenic differentiation of dental derived mesenchymal stem cells (MSCs). MSCs were encapsulated in an injectable and thermo‐responsive cell carrier (Pluronic ® F‐127) loaded with rhBMP4 and then photoactivated. PBM improved MSCs self‐renewal and survival upon encapsulation in the Pluronic ® F‐127. In the presence of rhBMP4, cell odonto/osteogenic differentiation was premature and markedly improved in the photoactivated MSCs. An in vivo calvarial critical sized defect model demonstrated significant increase in bone formation after PBM treatment. Finally, a balance in the reactive oxygen species levels may be related to the favorable results of PBM and rhBMP4 association. PBM may act in synergism with rhBMP4 and is a promise candidate to direct and accelerate hard tissue bioengineering.