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RNA binding protein Musashi‐2 regulates PIWIL1 and TBX1 in mouse spermatogenesis
Author(s) -
Sutherland Jessie M.,
Sobinoff Alexander P.,
Fraser Barbara A.,
Redgrove Kate A.,
Siddall Nicole A.,
Koopman Peter,
Hime Gary R.,
McLaughlin Eileen A.
Publication year - 2018
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.26168
Subject(s) - biology , microbiology and biotechnology , transcription factor , germ cell , rna binding protein , tbx1 , gene expression , regulation of gene expression , transcriptional regulation , gamete , microarray analysis techniques , downregulation and upregulation , translational regulation , gene , messenger rna , genetics , translation (biology) , sperm , promoter
RNA‐binding proteins (RBP) are important facilitators of post‐transcriptional gene regulation. We have previously established that nuclear overexpression of the RBP Musashi‐2 (MSI2) during male germ cell maturation is detrimental to sperm cell development and fertility. Herein we determine the genes and pathways impacted by the upregulation of Msi2 . Microarray analysis and qPCR confirmed differential gene expression in factors fundamental to the cell cycle, cellular proliferation, and cell death. Similarly, comparative protein expression analysis via iTRAQ, immunoblot, and immunolocalization, identified differential expression and localization of important regulators of transcription, translation, RNA processing, and spermatogenesis. Specifically, the testis‐expressed transcription factor, Tbx1 , and the piRNA regulator of gamete development, Piwil1 , were both found to be targeted for translational repression by MSI2. This study provides key evidence to support a fundamental role for MSI2 in post‐transcriptional regulation during male gamete development.