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Agarose hydrogel induced MCF‐7 and BMG‐1 cell line progressive 3D and 3D revert cultures
Author(s) -
Subramaniyan Aishwarya,
Ravi Maddaly
Publication year - 2018
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.25965
Subject(s) - agarose , cell culture , 3d cell culture , self healing hydrogels , scaffold , in vitro , microbiology and biotechnology , in vivo , mcf 7 , matrix (chemical analysis) , chemistry , cancer cell , biophysics , biology , cancer , biomedical engineering , biochemistry , medicine , genetics , organic chemistry , chromatography , human breast
3D culture systems have enhanced the utility of cancer cell lines as they are considered closer to the in vivo systems. A variety of changes are induced in cells cultured in 3D systems; an apparent and striking feature being the spontaneous acquisition of distinct morphological entities. 3D reverts (3DRs) can be obtained by introducing 3D aggregates in scaffold/matrix‐free culture units. It could be seen that the two cell lines used in this study exhibited differences in 3DR structures, though both were cultured on agarose hydrogels. Also, differences in 3DR formation, growth and survival were different. While 3D aggregates of several cell lines have been reported for a variety of studies, there are no studies that describe or utilize 3DRs. 3DRs can provide insights into complex events that can occur in cancer cells; especially as material to study metastasis, migration, and invasion.