Premium
Mesenchymal stem cells improves survival in LPS‐induced acute lung injury acting through inhibition of NETs formation
Author(s) -
Pedrazza Leonardo,
Cunha Aline Andrea,
Luft Carolina,
Nunes Nailê Karine,
Schimitz Felipe,
Gassen Rodrigo Benedetti,
Breda Ricardo Vaz,
Donadio Marcio Vinícius Fagundes,
de Souza Wyse Angela Terezinha,
Pitrez Paulo Marcio Condessa,
Rosa Jose Luis,
de Oliveira Jarbas Rodrigues
Publication year - 2017
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.25816
Subject(s) - mesenchymal stem cell , ards , proinflammatory cytokine , inflammation , medicine , lung , parenchyma , lipopolysaccharide , neutrophil extracellular traps , oxidative stress , immunology , pharmacology , pathology
Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are syndromes of acute hypoxemic respiratory failure resulting from a variety of direct and indirect injuries to the gas exchange parenchyma of the lungs. During the ALI, we have an increase release of proinflammatory cytokines and high reactive oxygen species (ROS) formation. These factors are responsible for the release and activation of neutrophil‐derived proteases and the formation of neutrophil extracellular traps (NETs). The excessive increase in the release of NETs cause damage to lung tissue. Recent studies have studies involving the administration of mesenchymal stem cells (MSCs) for the treatment of experimental ALI has shown promising results. In this way, the objective of our study is to evaluate the ability of MSCs, in a lipopolysaccharide (LPS)‐induced ALI model, to reduce inflammation, oxidative damage, and consequently decrease the release of NETs. Mice were submitted lung injury induced by intratracheal instillation of LPS and subsequently treated or not with MSCs. Treatment with MSCs was able to modulate pulmonary inflammation, decrease oxidative damage, and reduce the release of NETs. These benefits from treatment are evident when we observe a significant increase in the survival curve in the treated animals. Our results demonstrate that MSCs treatment is effective for the treatment of ALI. For the first time, it is described that MSCs can reduce the formation of NETs and an experimental model of ALI. This finding is directly related to these cells modulate the inflammatory response and oxidative damage in the course of the pathology.