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Biological roles of glial fibrillary acidic protein as a biomarker in cartilage regenerative medicine
Author(s) -
Kanazawa Sanshiro,
Nishizawa Satoru,
Takato Tsuyoshi,
Hoshi Kazuto
Publication year - 2017
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.25771
Subject(s) - glial fibrillary acidic protein , cartilage , regenerative medicine , microbiology and biotechnology , regeneration (biology) , cell , pathology , matrix (chemical analysis) , biology , chemistry , anatomy , stem cell , immunohistochemistry , medicine , biochemistry , chromatography
Glial fibrillary acidic protein (GFAP) is an intermediate filament that is expressed in specifically expressed auricular chondrocytes, which are good cell sources of cartilage regenerative medicine. Although our group uses GFAP as a biomarker of matrix production in the cultured auricular chondrocytes, the biological roles of GFAP in auricular chondrocytes has remained unknown. In this study, we demonstrated the biological functions of GFAP in the human and mouse derived auricles to clarify the significance and role with the chondrocytes of GFAP in order to provide useful information for reliable and safe regenerative medicine. We examined the cell responses to stretch stress for these chondrocytes and completed a nuclear morphological analysis. Based on these results, GFAP seems to support the resistance to severe mechanical stress in the tissue which physiologically suffers from a stretch overload, and plays pivotal roles in the conservation of cell structures and functions through the maintenance of nuclear morphology.