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Prototype of Multiplex Bead Assay for Quantification of Three Serum Biomarkers for In Vitro Diagnosis of Endometriosis
Author(s) -
Signorile Pietro Giulio,
Baldi Alfonso
Publication year - 2016
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.25410
Subject(s) - endometriosis , multiplex , medicine , disease , in vitro , pathology , microbiology and biotechnology , biology , bioinformatics , biochemistry
Endometriosis is a very common disease, affecting 10% of women in the reproductive age. To date, a significant delay between onset of the symptoms and definitive diagnosis is caused by the lack of a reliable non‐invasive diagnostic test. Recently, the potential value as diagnostic markers for endometriosis of three proteins (Zn‐alpha2‐glycoprotein, serum albumin, and complement C3 precursor), has been showed. In this article, we have defined the experimental conditions for the development of a multiplex bead array assay for rapid and simultaneous quantification of these three biomarkers in the serum of patients with endometriosis. Finally, pivotal experiments on a small cohort of patients have confirmed the diagnostic value of this assay. J. Cell. Physiol. 231: 2622–2627, 2016. © 2016 Wiley Periodicals, Inc.