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The Lineage Specification of Mesenchymal Stem Cells Is Directed by the Rate of Fluid Shear Stress
Author(s) -
Lu Juan,
Fan Yijuan,
Gong Xiaoyuan,
Zhou Xin,
Yi Caixia,
Zhang Yinxing,
Pan Jun
Publication year - 2016
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.25278
Subject(s) - mesenchymal stem cell , mechanosensitive channels , chondrogenesis , calcium , microbiology and biotechnology , intracellular , chemistry , calcium in biology , biophysics , actin , biology , biochemistry , receptor , ion channel , organic chemistry
The effective regulation of fluid shear stress (FSS) on the lineage specification of mesenchymal stem cells (MSCs) remains to be addressed. We hypothesized that when MSCs are recruited to musculoskeletal system following stimulation, their differentiation into osteogenic or chondrogenic cells is directed by the rate of FSS (ΔSS) through modulation of the mechanosensitive, cation‐selective channels (MSCCs), intracellular calcium levels, and F‐actin. To this end, MSCs were exposed to laminar FSS linearly increased from 0 to 10 dyn/cm 2 in 0, 2, or 20 min and maintained at 10 dyn/cm 2 for a total of 20 min (termed as ΔSS 0‐0′, 0‐2′, and 0‐20′, respectively, representing more physiological (0‐0′) and non‐physiological (0‐2′ and 0‐20′) ΔSS treatments). Our results showed 0‐0′ facilitated MSC differentiation towards chondrogenic and not osteogenic phenotype, by promoting moderate intracellular calcium concentration ([Ca 2+ ] i ) increase from the calcium channels with the exception of MSCCs or intracellular calcium stores, and F‐actin organization. In contrast, 0‐2′ promoted MSCs towards osteogenic and not chondrogenic phenotype, by inducing significant [Ca 2+ ] i increase mainly from the MSCCs, and F‐actin assembly. However, 0‐20′ elicited the modest osteogenic and chondrogenic phenotypes, as it induced the lowest [Ca 2+ ] i increase mainly from MSCCs, and F‐actin assembly. Our results suggest that compared to the more physiological ΔSS, the non‐physiological ΔSS favors [Ca 2+ ] i influx from MSCCs. An appropriate non‐physiological ΔSS (0‐2′) even elicits a large [Ca 2+ ] i influx from the MSCCs that reverses the lineage specification of MSCs, providing validation for the high mechanosensitivity of MSCs and guidance for training osteoporosis and osteoarthritis patients. J. Cell. Physiol. 231: 1752–1760, 2016. © 2015 Wiley Periodicals, Inc.