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Thymosin β4 Prevents Angiotensin II‐Induced Cardiomyocyte Growth by Regulating Wnt/WISP Signaling
Author(s) -
Li Li,
Guleria Rakeshwar S.,
Thakur Suresh,
Zhang ChengLin,
Pan Jing,
Baker Kenneth M.,
Gupta Sudhiranjan
Publication year - 2016
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.25275
Subject(s) - wnt signaling pathway , angiotensin ii , muscle hypertrophy , cell growth , endocrinology , microbiology and biotechnology , medicine , cell , signal transduction , biology , blot , chemistry , gene , biochemistry , blood pressure
Thymosin beta‐4 (Tβ4) is a ubiquitous protein with many properties relating to cell proliferation and differentiation that promotes wound healing and modulates inflammatory mediators. However, the role of Tβ4 in cardiomyocyte hypertrophy is currently unknown. The purpose of this study was to determine the cardio‐protective effect of Tβ4 in angiotensin II (Ang II)‐induced cardiomyocyte growth. Neonatal rat ventricular cardiomyocytes (NRVM) were pretreated with Tβ4 followed by Ang II stimulation. Cell size, hypertrophy marker gene expression and Wnt signaling components, β‐catenin, and Wnt‐induced secreted protein‐1 (WISP‐1) were evaluated by quantitative real‐time PCR, Western blotting and fluorescent microscopy. Pre‐treatment of Tβ4 resulted in reduction of cell size, hypertrophy marker genes and Wnt‐associated gene expression, and protein levels; induced by Ang II in cardiomyocyte. WISP‐1 was overexpressed in NRVM and, the effect of Tβ4 in Ang II‐induced cardiomyocyte growth was evaluated. WISP‐1 overexpression promoted cardiomyocytes growth and was reversed by pretreatment with Tβ4. This is the first report which demonstrates that Tβ4 targets Wnt/WISP‐1 to protect Ang II‐induced cardiomyocyte growth. J. Cell. Physiol. 231: 1737–1744, 2016. © 2015 Wiley Periodicals, Inc.