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C‐ing the Genome: A Compendium of Chromosome Conformation Capture Methods to Study Higher‐Order Chromatin Organization
Author(s) -
Barutcu A. Rasim,
Fritz Andrew J.,
Zaidi Sayyed K.,
van Wijnen André J.,
Lian Jane B.,
Stein Janet L.,
Nickerson Jeffrey A.,
Imbalzano Anthony N.,
Stein Gary S.
Publication year - 2016
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.25062
Subject(s) - chromatin , chromosome conformation capture , compendium , computational biology , biology , dna , genome , genomic organization , chromosome , chromosomal translocation , transcription (linguistics) , genetics , transcription factor , gene , enhancer , linguistics , philosophy , archaeology , history
Three‐dimensional organization of the chromatin has important roles in transcription, replication, DNA repair, and pathologic events such as translocations. There are two fundamental ways to study higher‐order chromatin organization: microscopic and molecular approaches. In this review, we briefly introduce the molecular approaches, focusing on chromosome conformation capture or “3C” technology and its derivatives, which can be used to probe chromatin folding at resolutions beyond that provided by microscopy techniques. We further discuss the different types of data generated by the 3C‐based methods and how they can be used to answer distinct biological questions. J. Cell. Physiol. 230: 31–35, 2016. © 2015 Wiley Periodicals, Inc.