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TSH/TSHR Signaling Suppresses Fatty Acid Synthase (FASN) Expression in Adipocytes
Author(s) -
Chen Jicui,
Ren Jianmin,
Jing Qingping,
Lu Sumei,
Zhang Yuchao,
Liu Yuantao,
Yu Cong,
Gao Peng,
Zong Chen,
Li Xia,
Wang Xiangdong
Publication year - 2015
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.24952
Subject(s) - fatty acid synthase , creb , endocrinology , medicine , kinase , protein kinase a , signal transduction , downregulation and upregulation , mapk/erk pathway , phosphorylation , chemistry , fatty acid synthesis , microbiology and biotechnology , lipid metabolism , biology , fatty acid , biochemistry , transcription factor , gene
TSH/TSHR signaling plays a role in the regulation of lipid metabolism in adipocytes. However, the precise mechanisms are not known. In the present study, we determined the effect of TSH on fatty acid synthase (FASN) expression, and explored the underlying mechanisms. In vitro, TSH reduced FASN expression in both mRNA and protein levels in mature adipocytes and was accompanied by protein kinase A (PKA) activation, cAMP‐response element binding protein (CREB) phosphorylation, as well as extracellular signal‐regulated kinase 1/2 (ERK1/2) and c‐Jun NH 2 ‐terminal kinase (JNK) activation. TSH‐induced downregulation of FASN was partially abolished by inhibition of PKA and ERK, but not JNK. TSHR and FASN expression in visceral tissue was significantly increased in C57BL/6 mice with diet‐induced obesity compared with control animals, whereas thyroid TSHR expression was normal. These findings suggest that activation of TSHR directly inhibits FASN expression in mature adipocytes, possibly mediated by PKA and ERK. In obese animals, this function of TSHR seems to be counteracted. The precise mechanisms need further investigation. J. Cell. Physiol. 230: 2233–2239, 2015. © 2015 Wiley Periodicals, Inc.