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Hairless Up‐Regulates Tgf‐β2 Expression via Down‐Regulation of miR‐31 in the Skin of “Hairpoor” ( Hr Hp ) Mice
Author(s) -
Kim BongKyu,
Yoon Sungjoo Kim
Publication year - 2015
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.24935
Subject(s) - hairless , hair follicle , derepression , hair cycle , microrna , transforming growth factor , keratinocyte , biology , inducer , medicine , microbiology and biotechnology , mutant , endocrinology , in vitro , chemistry , gene expression , gene , psychological repression , genetics
Hairless (HR) has been shown to regulate hair follicle (HF) morphogenesis and hair cycling. The Hr mutant hair loss mouse referred to as “hairpoor” ( Hr Hp ) displays overexpression of the HR protein through translational derepression. In this study, we found that 64 miRNAs were differentially expressed between the skin of Hr Hp / Hr Hp and wild type mice at P7 using miRNA‐microarray analysis and miR‐31 displayed the most reduced expression in Hr Hp / Hr Hp skin. In vivo observation and investigation using an in vitro reporter expression system revealed that miR‐31 and pri‐miR‐31 were consistently down‐regulated in the HR over‐expressed condition. In addition, we found that the transforming growth factor β 2 ( Tgf‐β2 ), a known catagen inducer, is the putative target of miR‐31. Furthermore, Tgf‐β2 level was also increased in HR over‐expressed keratinocyte and Hr Hp / Hr Hp mice. These study results suggest that HR controls Tgf‐β2 expression via regulation of miR‐31, thus causing abnormal hair cycle in Hr Hp / Hr Hp mice. J. Cell. Physiol. 230: 2075–2085, 2015. © 2015 Wiley Periodicals, Inc.