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Lipidomic approach towards deciphering anandamide effects in rat decidual cell
Author(s) -
Almada Marta,
Domingues M. Rosário,
Dória M. Luísa,
Fonseca Bruno M.,
Teixeira Natércia A.,
CorreiadaSilva Georgina
Publication year - 2015
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.24901
Subject(s) - anandamide , decidualization , endocannabinoid system , decidual cells , cardiolipin , lipidomics , phosphatidylserine , lipid signaling , chemistry , phospholipid , microbiology and biotechnology , apoptosis , lipid metabolism , sphingomyelin , placentation , medicine , endocrinology , biology , biochemistry , cannabinoid receptor , endometrium , placenta , pregnancy , fetus , receptor , membrane , genetics , agonist
Altered phospholipid (PL) metabolism has been associated with pregnancy disorders. Moreover, lipid molecules such as endocannabinoids (eCBs) and prostaglandins (PGs) are important mediators of reproductive events. In humans, abnormal decidualization has been linked with unexplained infertility, miscarriage and endometrial pathologies. Anandamide (AEA), the major eCB, induces apoptosis in rat decidual cells. In this study, the PL profile of rat decidual cells was characterized by a Mass spectrometry (MS) based lipidomic approach. Furthermore, we analyzed a possible correlation between changes in PL of rat decidual cells' membrane and AEA‐induced apoptosis. We found an increase in phosphatidylserine and a reduction of cardiolipin and phophatidylinositol relative contents. In addition, we observed an increase in the content of alkyl(alkenyl) acylPL, plasmalogens, and of long chain fatty acids especially with high degrees of unsaturation, as well as an increase in lipid hydroperoxides in treated cells. These findings provide novel insights on deregulation of lipid metabolism by anandamide, which may display further implications in decidualization process. J. Cell. Physiol. 230: 1549–1557, 2015. © 2014 Wiley Periodicals, Inc., A Wiley Company