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Quantifying heterogeneity and dynamics of clonal fitness in response to perturbation
Author(s) -
Frick Peter L.,
Paudel Bishal B.,
Tyson Darren R.,
Quaranta Vito
Publication year - 2015
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.24888
Subject(s) - biology , population , fitness landscape , evolutionary dynamics , evolutionary biology , clonal selection , genetic fitness , genetics , biological evolution , demography , sociology , immunology
The dynamics of heterogeneous clonal lineages within a cell population, in aggregate, shape both normal and pathological biological processes. Studies of clonality typically relate the fitness of clones to their relative abundance, thus requiring long‐term experiments and limiting conclusions about the heterogeneity of clonal fitness in response to perturbation. We present, for the first time, a method that enables a dynamic, global picture of clonal fitness within a mammalian cell population. This novel assay allows facile comparison of the structure of clonal fitness in a cell population across many perturbations. By utilizing high‐throughput imaging, our methodology provides ample statistical power to define clonal fitness dynamically and to visualize the structure of perturbation‐induced clonal fitness within a cell population. We envision that this technique will be a powerful tool to investigate heterogeneity in biological processes involving cell proliferation, including development and drug response. J. Cell. Physiol. 230: 1403–1412, 2015. © 2015 Wiley Periodicals, Inc., A Wiley Company