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Modulation of Mast Cell Proliferative and Inflammatory Responses by Leukotriene D 4 and Stem Cell Factor Signaling Interactions
Author(s) -
AlAzzam Nosayba,
Kondeti Vinay,
Duah Ernest,
Gombedza Farai,
Thodeti Charles K.,
Paruchuri Sailaja
Publication year - 2015
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.24777
Subject(s) - stem cell factor , mast cell , microbiology and biotechnology , stem cell , modulation (music) , cell , signal transduction , cell signaling , inflammation , biology , chemistry , immunology , biochemistry , physics , progenitor cell , acoustics
Mast cells (MCs) are important effector cells in asthma and pulmonary inflammation, and their proliferation and maturation is maintained by stem cell factor (SCF) via its receptor, c‐Kit. Cysteinyl leukotrienes (cys‐LTs) are potent inflammatory mediators that signal through CysLT 1 R and CysLT 2 R located on the MC surface, and they enhance MC inflammatory responses. However, it is not known if SCF and cys‐LTs cross‐talk and influence MC hyperplasia and activation in inflammation. Here, we report the concerted effort of the growth factor SCF and the inflammatory mediator LTD 4 in MC activation. Stimulation of MCs by LTD 4 in the presence of SCF enhances c‐Kit‐mediated proliferative responses. Similarly, SCF synergistically enhances LTD 4 ‐induced calcium, c‐fos expression and phosphorylation, as well as MIP1β generation in MCs. These findings suggest that integration of SCF and LTD 4 signals may contribute to MC hyperplasia and hyper‐reactivity during airway hyper‐response and inflammation. J. Cell. Physiol. 230: 595–602, 2015. © 2014 Wiley Periodicals, Inc., A Wiley Company