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Recovery and Cultivation of Keratinocytes From Shipped Mouse Skin
Author(s) -
Yang Hsinya,
La Thi Dinh,
Gurenko Zhanna,
Steenhuis Pieter,
Liu Wei,
Isseroff R. Rivkah
Publication year - 2015
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.24775
Subject(s) - keratinocyte , biology , transgene , genetically modified mouse , microbiology and biotechnology , cell culture , immunology , gene , biochemistry , genetics
Murine keratinocyte culture from neonatal skin is an important tool for studying the functional role of specific genes in epithelial biology. However, when the transgenic animal is only available in a geographically distant local, obtaining viable keratinocytes can be problematic. A method for transferring the isolated murine skin from collaborating labs could decrease the cost of shipping live animals, and would allow the efficient use of the tissues from the transgenic animals. Here we optimized shipping conditions and characterized the cells retrieved and cultured from mouse skin shipped for 48 h at 0 °C. The cultured keratinocytes from the control, non‐shipped skin and the 2‐day shipped skin were 43.6 +/− 7.8% viable, doubled every 2 days, and expressed comparable amounts of heat shock proteins and CD29/integrin beta‐1. However, under the same shipping conditions, the 3‐day shipped tissue failed to establish colonies in the culture. Therefore, this 2‐day shipping technique allows the transfer mouse skin from distant locations with recovery of viable, propagatable keratinocytes, facilitating long‐distance collaborations. J. Cell. Physiol. 230: 242–245, 2015. © 2014 Wiley Periodicals, Inc.