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Brain Natriuretic Peptide and C‐Type Natriuretic Peptide Maintain Porcine Oocyte Meiotic Arrest
Author(s) -
Zhang Wenqiang,
Yang Ye,
Liu Wei,
Chen Qian,
Wang Huarong,
Wang Xiao,
Zhang Yanhao,
Zhang Meijia,
Xia Guoliang
Publication year - 2015
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.24682
Subject(s) - npr2 , natriuretic peptide , oocyte , medicine , endocrinology , atrial natriuretic peptide , brain natriuretic peptide , receptor , npr1 , in vivo , biology , human chorionic gonadotropin , microbiology and biotechnology , hormone , embryo , heart failure
Recent studies have shown that C‐type natriuretic peptide (CNP) serves as a key control system during mouse oocyte maturation. We used pig models (in vitro and in vivo) to explore the role played by the natriuretic peptide family in porcine oocyte maturation. We reported the expression and location of natriuretic peptide system in different stages of porcine antral follicles. Atrial natriuretic peptide (ANP) and CNP were expressed primarily in granulosa cells, whereas brain natriuretic peptide (BNP) and natriuretic peptide receptor‐B (NPRB) receptor were expressed in granulosa cells (both cumulus and mural granulosa cells) and thecal internal cells, and the natriuretic peptide receptor‐A (NPRA) receptor predominantly in thecal cells. Upon in vitro culture, BNP and CNP maintained meiotic arrest of oocytes associated with cumulus cells. The expression levels of BNP, CNP, and the NPRB receptor increased upon treatment of prepubertal gilts with pregnant mare's serum gonadotropin and decreased upon subsequent human chorionic gonadotropin injection. Such dynamic changes in the expression of natriuretic peptides and their receptor paralleled the proportions of oocytes exhibiting nuclear maturation in vivo. These data indicated that BNP and CNP co‐contributed to maintaining porcine meiotic arrest under physiological condition and lutenizing hormone (LH) relieved this inhibitory effect by decreasing the expression levels of BNP and CNP in vivo. Our present work, combined with previous data, improved the understanding of the oocyte meiotic arrest mechanisms and further revealed that natriuretic peptides serve as oocyte maturation inhibitor (OMI) to inhibit oocyte maturation in mammals. J. Cell. Physiol. 230: 71–81, 2015. © 2014 Wiley Periodicals, Inc.