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The Dynamic Architectural and Epigenetic Nuclear Landscape: Developing the Genomic Almanac of Biology and Disease
Author(s) -
Tai Phillip W.L.,
Zaidi Sayyed K.,
Wu Hai,
Grandy Rodrigo A.,
Montecino Martin,
van Wijnen André J.,
Lian Jane B.,
Stein Gary S.,
Stein Janet L.
Publication year - 2014
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.24508
Subject(s) - epigenetics , biology , chromatin , context (archaeology) , computational biology , evolutionary biology , genome , cell nucleus , nucleus , gene , neuroscience , genetics , paleontology
Abstract Compaction of the eukaryotic genome into the confined space of the cell nucleus must occur faithfully throughout each cell cycle to retain gene expression fidelity. For decades, experimental limitations to study the structural organization of the interphase nucleus restricted our understanding of its contributions towards gene regulation and disease. However, within the past few years, our capability to visualize chromosomes in vivo with sophisticated fluorescence microscopy, and to characterize chromosomal regulatory environments via massively parallel sequencing methodologies have drastically changed how we currently understand epigenetic gene control within the context of three‐dimensional nuclear structure. The rapid rate at which information on nuclear structure is unfolding brings challenges to compare and contrast recent observations with historic findings. In this review, we discuss experimental breakthroughs that have influenced how we understand and explore the dynamic structure and function of the nucleus, and how we can incorporate historical perspectives with insights acquired from the ever‐evolving advances in molecular biology and pathology. J. Cell. Physiol. 229: 711–727, 2014. © 2013 Wiley Periodicals, Inc.